Laboratory of Advanced Microscopy

Confocal Microscopy


Confocal microscopy is nowadays a standard technology for high resolution observations of living or fixed biological samples. It allows volume reconstructions and time sequencing, two features that proved to be extremely powerful when applied to the study of living cells, tissues or organisms.

A confocal microscope differs from a traditional fluorescence microscope for the use of a LASER as a light source (which drastically improves image quality because of the higher intensity and coherence of the emitted light), and for the presence of an optical and electronical system that allows the optical scanning of a sample section (optical section) and its reconstruction on a computer display.

These features improve image quality (higher resolution and contrast, easier image acquisition and storage), but also opened the way to new applications of optical microscopy, such as three-dimensional reconstruction of the sample [clic here].

How does it work?

Useful links

Our lab has two confocal microscopes with different performances and applications.

A Bio-Rad ViewScan microscope, performing a line scanning of the sample and allowing direct observation and video-rate image acquisition.

An Olympus FluoView microscope, that can reach higher resolutions and thinner optical sections. It can therefore be used for many applications such as 3D-reconstructions, time sequencing or in situ fluorescence hybridisation (FISH).

A Leica TCS SC2 microscope, that can reach theorical resolution limits. It can be used for 3D-reconstructions, time sequencing, spectral scanning, FRAP and FRET

All our instruments are accessible by guest users, with the help of our technical staff. For questions and reservations, please read our fee list and contact: genre@bioveg.unito.it



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