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Confocal microscopy is nowadays a standard technology for high resolution observations of living or fixed biological samples. It allows volume reconstructions and time sequencing, two features that proved to be extremely powerful when applied to the study of living cells, tissues or organisms.
A confocal microscope differs from a traditional fluorescence microscope for the use of a LASER as a light source (which drastically improves image quality because of the higher intensity and coherence of the emitted light), and for the presence of an optical and electronical system that allows the optical scanning of a sample section (optical section) and its reconstruction on a computer display.
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